Multiparametric slice culture platform for the investigation of human cardiac physiology – Rose Yin

Human cardiac slices have recently emerged as a promising model of human cardiac physiology and pharmaceutical testing because it recapitulates organ level multi-cellular interactions and tissue architecture that traditional cell culture and animal models are not able to fully capture. Our lab has developed a method of collecting and culturing cardiac slices from human hearts rejected for organ transplant. To preserve the electrical and mechanical properties of these cardiac slices in long-term culture, we are developing an automated, microfluidic, fully-enclosed organotypic culture system that provides electrical, mechanical, and optical stimulation and real-time ECG monitoring to achieve long-term culture of cardiac slices.